Biotinylated Dextran Amine을 이용한 금화조
Song Control System의 뇌신경 회로
추적 - 신경망 연결과 Tonotopy |
임덕환 |
한림대학교 언어청각학부 청각학전공, 청각언어연구소 |
Tracing Song Control System of the Zebra Finch with
Biotinylated Dextran Amine - Neural Connection
and Tonotopic Arrangement |
Dukhwan Lim |
Section of Audiology, Audiology and Speech Pathology Research Institute, Hallym University, Chuncheon, Korea |
Correspondence |
Dukhwan Lim ,Tel: (033) 248-2217, Fax: (02) 6280-9133, Email: dlim@hallym.ac.kr
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Received: February 8, 2006; Accepted: April 23, 2006. Published online: June 30, 2006. |
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ABSTRACT |
Auditory neural tracts were examined in the male zebra finch (Poephila guttata) to understand how the nuclei of the song control
system connect each other to process complex auditory features. First, creysel violet and biotinylated dextran amine (BDA) were
used as both anterograde and retrograde tracers. BDA tracer was injected in the HVc (N=3). After a survival time of 5 to 7 days, the
brain of the animal was traced in both retrograde and anterograde directions. Second, subjects were stimulated with the pure tone of 1
kHz during the survival time to trace the tonotopy in Ov after the BDA tracer was injected in Field L (N=2). The results showed
that dense neural connections between HVc and RA. Typical band shaped frequency regions were identified in Ov in response to 1 kHz
pure tone stimulation in Field L injection. Based on these findings in anatomical tracing, sensory and motor subdivisions appeared to
interact with each other and typical pure tone tonotopy was preserved at least up to the stage of Ov. |
Key Words:
Biotinylated dextran amine·Song control system·Zebra finch·Selectivity. |
중심단어:
Biotinylated dextran amine· Song control system· 금화조· 선택도. |
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